抗體製備服務

從基因表現到抗體製備從多肽合成到抗體製備

專業的服務與產品滿足在乎品質的您~~

原廠特色

經驗豐富：合作廠商有超過12年以上的經驗，目前同時供應來自超過四、五十幾個國家大廠的訂單！
簽定合約 : 每一件代工，都需簽定合約，保證客戶的權益。
保密性 : 對任何一件代工，我們將為您嚴格保密。如果客戶需要，我們可以與客戶簽定保密協定。
收件彈性大：從客戶的任何實驗過程都可接手而有不同計費。
價格合理：國外接單處理，國內價格！
融合瘤保留 : 客戶完成的單元抗體案發件後，除了提供給客戶2-5個融合瘤細胞株外，合作原廠也會幫客戶保存一年。

交付成品

a) 0.5-1 ml pre-immune sera。

b) 60-80 ml antisera or 2-5 mg purified antibody

c) Test report (ELISA data, Western Blot…etc)

d) 2-3 mg peptides (unmodified & phospho) (★Peptide synthesis only) 。

e) 2-5 DNA clones and Recombinant vector (★ Gene expression only) 。

f ) 2-5 clones of hybridoma cell and isotype determination result (★ Monoclone only) 。

g) project report。

基因表現&多肽合成到抗體製備的流程

從基因表現到抗體製備

以(cat#: GSM-004) Monoclonal package 4 (From cDNA to ascites purification)為例
過程	實驗項目	所需時間	客戶自行進行	GeneMark服務
Gene amplification	RT-PCR or PCR kit (proof-reading enzymes) 引子合成電泳分析 DNA回收 DNA定量	1 week	RT-PCR kit or PCR kit 約$3000 primer合成約$1000-1500 PCR clean-Up or gel elution kit 約$900	不用!
Cloning	Expression vector之選擇與購買 DNA黏合及clone 篩選	1 week	Expression vector 約$9000 ligation及Screen費用約$2000	不用!
Restirction enzyme or sequencing identification	R.E酵素剪切確認定序	1 week	酵素切及電泳保守估計$1000 定序費用約$3000	不用!
Gene Express and protein purification	基因表現蛋白質結合與溶離 PAGE分析及蛋白質定量	2 weeks	His binding resin約$1000 電泳分析及buffer約$500	不用!
Immunization, Hybridoma development and screening	免疫前血清及第一次免疫動物 3 次免疫注射動物血清採集及ELISA測試融合瘤技術及初部篩選 positive clones	10-13 weeks	動物花費約 $1000 ELISA測試花費約$2000 Hybridization花費約$2000	不用!
Subcloning Expand hybridoma and monoclonal antibody production	一次、二次、三次篩選 ELISA 測試所篩選的subclone 大量培養hybridoma Cells ELISA 測試細胞 supernant;確定其positive clones	8-10 week	Medium, 耗材花費約 $5000 ELISA測試花費約 $2000	不用!
Identification Affinity purified antibody	抗體Iso-typing 分析免疫老鼠得到腹水腹水抗體純化及ELISA測試	4-5 weeks	Isotyping kit 約$12000 免疫動物得到腹水抗體約 $500 ELISA測試約 $2000 抗體純化約 $6000	不用!
期間人力工時之消耗	助理之工資工時之消耗耗材之消耗儀器之消耗人為錯誤之消耗		1. 助理最少需24 個星期，支出約$216000(助理以$36000/月計) 2. 儀器及耗材消耗保守估計$20000-30000	不用!
服務費	無		0	$195,000
合計			>$280,000	$195,000
實驗做不好	所有過程重新再來一遍		重來，得再花一次同樣的費用	原廠負責

訂購方式

服務項目

Polyclone Antibody
產品編號	服務項目	服務工作	提供客戶成品	時間	價格
GSP001	Polyclonal package 1 (From protein to antisera)	protein checked by SDS-PAGE immunization and sera collection ELISA identification	0.5-1 ml pre-immune sera 60-80 ml antisera ELISA result (>1:12000) project report	10-12 wks	$17,000
GSP002	Polyclonal package 2 (From protein to antigen purified antibodies)	protein checked by SDS-PAGE immunization and sera collection ELISA identification antigen affinity purification	0.5-1 ml pre-immune sera 2-5 mg purified antibodies ELISA result (.1:32000) project report	10-12 wks	$23,000
GSP003	Polyclonal package 3 (From peptide design to antisera)	peptide design and synthesis(12-22aa,85%) peptide conjugation immunization and sera collection ELISA identification	2-3mg peptides 0.5-1 ml pre-immune sera/rabbit 60-80 ml antisera ELISA result (>1:12000) project report	12-15 wks	$29,000
GSP004	Polyclonal package 4 (From peptide design to antigen purified antibodies)	1 peptide design and synthesis(12-22aa,85%) peptide conjugation immunization and sera collection ELISA identification antigen affinity purification	2-3 mg peptides 0.5-1 ml pre-immune sera 2-5 mg purified antibodies ELISA result (>1:32000) project report	12-15 wks	$34,000
GSP005	Polyclonal package 5 (From plasmid to antisera)	protein expression and purification immunization and sera collection ELISA identification	1 mg protein 0.5-1 ml pre-immune sera/rabbit 60-80 ml antisera ELISA result (>1:12000) project report	12-15 wks	$36,000
GSP006	Polyclonal package 6 (From plasmid to antigen purified antibodies)	protein expression and purification immunization and sera collection ELISA identification antigen affinity purification	1 mg protein (purity>90%) 0.5-1 ml pre-immune sera/rabbit 2-5 mg purified antibodies ELISA result (>1:32000) project report	12-15 wks	$41,000
GSP007	Polyclonal package 7 (From cDNA to antisera)	gene amplification vector construction protein expression and purification immunization and sera collection ELISA identification	recombinant vector 1 mg protein (purity>90%) 0.5-1 ml pre-immune sera/rabbit 60-80 ml antisera ELISA result (>1:12000) project report	14-16 wks	$63,000
GSP008	Polyclonal package 8 (From cDNA to antigen purified antibodies)	gene amplification vector construction protein expression and purification immunization and sera collection ELISA identification antigen affinity purification	recombinant vector 1 mg protein (purity>90%) 0.5-1 ml pre-immune sera/rabbit 2-5 mg purified antibodies ELISA result (>1:32000) project report	14-16 wks	$68,000
GSP009	Polyclonal package 9 (Phospho- specific polyclonal antibodies)	1 peptide design and synthesis(12-22aa,85%) 1 Phospho-specific site peptide conjugation immunization and sera collection ELISA identification antigen affinity purification	2-3 mg peptides (unmodified & phospho) 0.5-1 ml pre-immune sera/rabbit 2-5 mg phospho-specific antibodies 3-5 mg non-phospho-specfic antibodies ELISA result (>1:32000, phospho-antibody) project report	13-16 wks	$70,000
GSP010	Polyclonal package 10 (Western guaranteed polyclonal antibodies)	2 peptide design and synthesis(12-22aa,85%) peptide conjugation immunization and sera collection ELISA & Western Blot identification antigen affinity purification	2-3 mg peptides 0.5-1 ml pre-immune sera/rabbit 2-5 mg purified antibodies ELISA & Western Blot results ELISA>1:32000, WB>1:200 At least 1 peptide’s antibody guaranteed project report	15-18 wks	$79,000
GSP011	Polyclonal package 11 (Western and IHC guaranteed polyclonal antibodies)	3 peptide design and synthesis(12-22aa,85%) peptide conjugation immunization and sera collection ELISA identification Western Blot & IHC identification antigen affinity purification	2-3 mg peptides 0.5-1 ml pre-immune sera/rabbit 2-5 mg purified antibodies ELISA result, Western Blot & IHC results ELISA>1:32000; WB.1:200 At least 1 peptide’s antibody guaranteed project report	15-18 wks	$87,000


Monoclone Antibody
產品編號	服務項目	服務工作	提供客戶成品	時　間	價　格
GSM001	Monoclonal package 1 (From protein to ascites purification)	protein checked by SDS-PAGE immunization and sera collection ELISA identification fusion and hybridoma development isotype determination Subcloning and cell cryopreservation ascites production and purification (2 clones)	50 ul pre-immune sera (1:10dilution) 2-5 hybridomas 2-5 mg protein A/G purified antibodies (2 clones) ELISA result isotype determination result project report	18-24 wks	$150,000
GSM002	Monoclonal package 2 (From peptide design to ascites purification)	peptide design and synthesis(12-22aa,85%) peptide conjugation immunization and sera collection ELISA identification fusion and hybridoma development isotype determination Subcloning and cell cryopreservation ascites production and purification (2 clones)	2-3 mg peptides 50 ul pre-immune sera (1:10dilution) 2-5 hybridomas 2-5 mg purified antibodies (2 clones) ELISA result (>1:80000) isotype determination result project report	20-26 wks	$165,000
GSM003	Monoclonal package 3 (From plasmid to ascites purification)	protein expression and purification immunization and sera collection ELISA identification fusion and hybridoma development isotype determination Subcloning and cell cryopreservation ascites production and purification (2 clones)	1 mg protein 50 ul pre-immune sera (1:10dilution) 2-5 clones 2-5 mg purified antibodies (2 clones) ELISA result (>1:80000) isotype determination result project report	20-26 wks	$175,000
GSM004	Monoclonal package 4 (From cDNA to ascites purification)	gene amplification vector construction protein expression and purification immunization and sera collection ELISA identification fusion and hybridoma development isotype determination Subcloning and cell cryopreservation ascites production and purification (2 clones)	recombinant vector 1 mg protein 50 ul pre-immune sera (1:10dilution) 2-5 clones 2-5 mg purified antibodies (2 clones) ELISA result (>1:80000) isotype determination result project report	22-26 wks	$195,000
GSM005	Monoclonal package 5 (Phospho- specific monoclonal antibodies)	1 peptide design and synthesis(12-22aa,85%) (unmodified&phospho) peptide conjugation immunization and sera collection ELISA identification fusion and hybridoma development isotype determination Subcloning and cell cryopreservation ascites production and purification (2 clones)	2-3 mg peptides (unmodified & phospho) 50 ul pre-immune sera (1:10dilution) 2-5 clones 2-5mg purified antibodies (2 clones) ELISA result (>1:80000) isotype determination result project report	21-26 wks	$220,000
GSM006	Monoclonal package 6 (Western guaranteed monoclonal antibodies)	2-3 peptide design and synthesis(12-22aa,85%) peptide conjugation immunization and sera collection ELISA & Western Blot identification fusion and hybridoma development isotype determination Subcloning and cell cryopreservation ascites production and purification (2 clones)	2-3 mg peptides 50 ul pre-immune sera (1:10dilution) 2-5 clones 2-5 mg purified antibodies (2 clones) ELISA & Western Blot results At least 1 peptide’s antibody guaranteed (ELISA>1:80000; WB>1:500) isotype determination result project report	20-26 wks	$280,000

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