抗體製備服務 Custom DNA Oligos Synthesis

 

 

從基因表現到抗體製備 從多肽合成到抗體製備 專業的服務與產品   滿足在乎品質的您~~
原廠特色
  • 經驗豐富:合作廠商有超過12年以上的經驗,目前同時供應來自超過四、五十幾個國家大廠的訂單!
  • 簽定合約 : 每一件代工,都需簽定合約,保證客戶的權益。
  • 保密性 : 對任何一件代工,我們將為您嚴格保密。如果客戶需要,我們可以與客戶簽定保密協定。
  • 收件彈性大:從客戶的任何實驗過程都可接手而有不同計費。
  • 價格合理:國外接單處理,國內價格!
  • 融合瘤保留 : 客戶完成的單元抗體案發件後,除了提供給客戶2-5個融合瘤細胞株外,合作原廠也會幫客戶保存一年。

 

交付成品

a) 0.5-1 ml pre-immune sera。

b) 60-80 ml antisera or 2-5 mg purified antibody

c) Test report (ELISA data, Western Blot…etc)

d) 2-3 mg peptides (unmodified & phospho) (★Peptide synthesis only) 。

e) 2-5 DNA clones and Recombinant vector   (★ Gene expression only) 。

f ) 2-5 clones of hybridoma cell and isotype determination result  (★ Monoclone only) 。

g) project report。

 

基因表現&多肽合成到抗體製備的流程

基因表現&多肽合成到抗體製備的流程

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從基因表現到抗體製備
超級比一比,交給GeneMark  VS. 客戶自行基因選殖及生產抗體
 
以(cat#: GMA-M04) Monoclonal package 4 (From cDNA to ascites purification)為例

過 程

實驗項目

所需時間

客戶自行進行

 GeneMark服務

Gene amplification RT-PCR or PCR kit (proof-reading enzymes) 引子合成 電泳分析 DNA回收 DNA定量 1 week RT-PCR kit or PCR kit 約$3600 primer合成約$1200-1800 PCR clean-Up or gel elution kit 約$900   不用!
Cloning Expression vector之選擇與購買 DNA黏合及clone 篩選 1 week Expression vector 約$9000 ligation及Screen費用約$2000 不用!
Restirction enzyme or sequencing identification R.E酵素剪切確認 定序 1 week 酵素切及電泳保守估計$1000 定序費用約$3000 不用!
Gene Express and protein purification 基因表現 蛋白質結合與溶離 PAGE分析及蛋白質定量 2 weeks His binding resin約$1000 電泳分析及buffer約$500 不用!
Immunization, Hybridoma development and screening 免疫前血清及第一次免疫動物 3 次免疫注射動物 血清採集及ELISA測試 融合瘤技術及初部篩選 positive clones 10-13 weeks 動物花費約 $1000 ELISA測試花費約$2000 Hybridization花費約$2000 不用!
Subcloning Expand hybridoma and monoclonal antibody production 一次、二次、三次篩選 ELISA 測試所篩選的subclone 大量培養hybridoma Cells ELISA 測試細胞 supernant;確定其positive clones 8-10 week Medium, 耗材花費約 $5000 ELISA測試花費約 $2000 不用!
Identification Affinity purified antibody 抗體Iso-typing 分析 免疫老鼠得到腹水 腹水抗體純化及ELISA測試 4-5 weeks Isotyping kit 約$12000 免疫動物得到腹水抗體約 $500 ELISA測試約 $2000 抗體純化約 $6000 不用!
期間人力工時之消耗 助理之工資 工時之消耗 耗材之消耗 儀器之消耗 人為錯誤之消耗   1. 助理最少需24 個星期,支出約$216000(助理以$36000/月計)
2. 儀器及耗材消耗保守估計$20000-30000
不用!
服務費   0 $234,000
合計   >$300,000 $234,000
實驗做不好 所有過程重新再來一遍   重來,得再花一次同樣的費用 原廠負責
         

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訂購方式

聯絡各區辦公室, 專人為您服務, 同時提供專案企書。

 

服務項目
Polyclone Antibody
產品編號 服務項目 服務工作 提供客戶成品 時間 價格
GMA-P01 Polyclonal package 1 (From protein to antisera) protein checked by SDS-PAGE immunization and sera collection ELISA identification 0.5-1 ml pre-immune sera 60-80 ml antisera ELISA result (>1:12000) project report 10-12 wks $20,400
GMA-P02 Polyclonal package 2 (From protein to antigen purified antibodies) protein checked by SDS-PAGE immunization and sera collection ELISA identification antigen affinity purification 0.5-1 ml pre-immune sera 2-5 mg purified antibodies ELISA result (.1:32000) project report 10-12 wks $27,600
GMA-P03 Polyclonal package 3 (From peptide design to antisera) peptide design and synthesis(12-22aa,85%) peptide conjugation immunization and sera collection ELISA identification 2-3mg peptides 0.5-1 ml pre-immune sera/rabbit 60-80 ml antisera ELISA result (>1:12000) project report 12-15 wks $34,800
GMA-P04 Polyclonal package 4 (From peptide design  to antigen purified antibodies) 1 peptide design and synthesis(12-22aa,85%) peptide conjugation immunization and sera collection ELISA identification antigen affinity purification 2-3 mg peptides 0.5-1 ml pre-immune sera 2-5 mg purified antibodies ELISA result (>1:32000) project report 12-15 wks $40,800
GMA-P05 Polyclonal package 5 (From plasmid  to antisera) protein expression and purification immunization and sera collection ELISA identification 1 mg protein 0.5-1 ml pre-immune sera/rabbit 60-80 ml antisera ELISA result (>1:12000) project report 12-15 wks $43,200
GMA-P06 Polyclonal package 6 (From plasmid  to antigen purified antibodies) protein expression and purification immunization and sera collection ELISA identification antigen affinity purification 1 mg protein (purity>90%) 0.5-1 ml pre-immune sera/rabbit 2-5 mg purified antibodies ELISA result (>1:32000) project report 12-15 wks $49,200
GMA-P07 Polyclonal package 7 (From cDNA  to antisera) gene amplification vector construction protein expression and purification immunization and sera collection ELISA identification recombinant vector 1 mg protein (purity>90%) 0.5-1 ml pre-immune sera/rabbit 60-80 ml antisera ELISA result (>1:12000) project report 14-16 wks $75,600
GMA-P08 Polyclonal package 8 (From cDNA to antigen purified antibodies) gene amplification vector construction protein expression and purification immunization and sera collection ELISA identification antigen affinity purification recombinant vector 1 mg protein (purity>90%) 0.5-1 ml pre-immune sera/rabbit 2-5 mg purified antibodies ELISA result (>1:32000) project report 14-16 wks $81,600
GMA-P09 Polyclonal package 9 (Phospho-  specific polyclonal antibodies) 1 peptide design and synthesis(12-22aa,85%) 1 Phospho-specific site peptide conjugation immunization and sera collection ELISA identification antigen affinity purification 2-3 mg peptides (unmodified & phospho) 0.5-1 ml pre-immune sera/rabbit 2-5 mg phospho-specific antibodies 3-5 mg non-phospho-specfic antibodies ELISA result (>1:32000, phospho-antibody) project report 13-16 wks $84,000
           

 

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Monoclone Antibody
產品編號 服務項目 服務工作 提供客戶成品 時 間 價 格
GMA-M01 Monoclonal package 1 (From protein to ascites purification) protein checked by SDS-PAGE immunization and sera collection ELISA identification fusion and hybridoma development isotype determination Subcloning and cell cryopreservation ascites production and purification (2 clones) 50 ul pre-immune sera (1:10dilution) 2-5 hybridomas 2-5 mg protein A/G purified antibodies (2 clones) ELISA result isotype determination result project report 18-24 wks $180,000
GMA-M02 Monoclonal package 2 (From peptide design  to ascites purification) peptide design and synthesis(12-22aa,85%) peptide conjugation immunization and sera collection ELISA identification fusion and hybridoma development isotype determination Subcloning and cell cryopreservation ascites production and purification (2 clones) 2-3 mg peptides 50 ul pre-immune sera (1:10dilution) 2-5 hybridomas 2-5 mg purified antibodies (2 clones) ELISA result (>1:80000) isotype determination result project report 20-26 wks $198,000
GMA-M03 Monoclonal package 3 (From plasmid to ascites purification) protein expression and purification immunization and sera collection ELISA identification fusion and hybridoma development isotype determination Subcloning and cell cryopreservation ascites production and purification (2 clones) 1 mg protein 50 ul pre-immune sera (1:10dilution) 2-5 clones 2-5 mg purified antibodies (2 clones) ELISA result (>1:80000) isotype determination result project report 20-26 wks $210,000
    GMA-M04   Monoclonal package 4 (From cDNA to ascites purification) gene amplification vector construction protein expression and purification immunization and sera collection ELISA identification fusion and hybridoma development isotype determination Subcloning and cell cryopreservation ascites production and purification (2 clones) recombinant vector 1 mg protein 50 ul pre-immune sera (1:10dilution) 2-5 clones 2-5 mg purified antibodies (2 clones) ELISA result (>1:80000) isotype determination result project report 22-26 wks $234,000
    GMA-M05  Monoclonal package 5 (Phospho-  specific monoclonal antibodies) 1 peptide design and synthesis(12-22aa,85%) (unmodified&phospho) peptide conjugation immunization and sera collection ELISA identification fusion and hybridoma development isotype determination Subcloning and cell cryopreservation ascites production and purification (2 clones) 2-3 mg peptides (unmodified & phospho) 50 ul pre-immune sera (1:10dilution) 2-5 clones 2-5mg purified antibodies (2 clones) ELISA result (>1:80000) isotype determination result project report 21-26 wks $264,000
           

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