2-4-1 pGM-T PCR Cloning Kit

The pGM-T Vectors are linearized vectors with a single3´-terminal thymidine at both ends. The T-overhangs at the insertion site greatly improve the efficiency of ligation of PCR products by preventing recircularization of the vector and providing a compatible overhang for PCR products generated by certain thermostable polymerases.

The pGM-T Vectors are high-copy-number vectors containing T7 and SP6 RNA polymerase promoters flanking a multiple cloning region within the α-peptide coding region of the enzyme β-galactosidase. Insertion a linactivation of the α-peptide allows identification of recombinants by blue/white screening on indicator plates.




 

One-Step RT-PCR Kit

產品資訊

《快速簡單》

  • 載體帶有T7及SP6起動子, 可容易進行in vitro transcription 及sequencing 。
  • 以X-Gal及IPTG (藍白篩選), 含insert插入者colonies呈現白色, 便於篩選。
  • 使用Ampicillin篩選。


《好用、高效率》

  • 選殖過程在1小時內完成。
  • 載體帶有單一EcoR I切位, 容易轉殖 (subclone)。


《經濟方便》

  • 使用者只要準備PCR產物即可, 不需額外準備其他材料, 減少額外花費。
  • 直接選殖PCR產物 (0.1-4 kb) 至載體中。

                   

 

 

 

產品內容物
   
1 vial of 20 μl pGM-T Vector (50 ng/μl)
1 vial of 01 μl T4 DNA ligase (3 U/μl)
1 vial of 30 μl 10X T4 DNA Ligation Buffer
1 vial of 100 μl 2X T4 Rapid Ligation Buffer
1 vial of 5 μl Control Fragment (300 bp)
21 vial of 100 μl DH5α Competent Cell
1 vials 5 μl Control Plasmid
1 vial of 400 μl Colony primer pairs
1 vial of 15 ml SOC medium
1 vial of 300 μl Nuclease-free water 

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pGM-T PCR Cloning Kit (not including competent cell)

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pGM-T vector 

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GM DNA Ligation Kit

 

訂購資訊
     
Products: Cat. No. Size
pGM-T PCR Cloning Kit GVT-202 20 r

pGM-T PCR Cloning Kit (not including competent cell)

GVT-202-0 20 r
pGM-T vector GVT-202-T1 20 r
GVT-202-T2 60 r
GM DNA Ligation Kit

GVT-202-L

20 r
     

 

上層分類:2 - PCR Related Products